71725 / 71729 Sodium dodecyl sulfate

SDS has a wide variety of applications, but is most often used as a protein and lipid solubilization reagent. As a general rule for the solubilization of proteins, SDS should …Web

قرأ أكثر

Thermodynamics of Sodium Dodecyl Sulfate Partitioning into Lipid

The partition equilibria of sodium dodecyl sulfate (SDS) and lithium dodecyl sulfate between water and bilayer membranes were investigated with isothermal titration calorimetry and spectroscopic methods (light scattering, 31 P-nuclear magnetic resonance) in the temperature range of 28°C to 56°C. The partitioning of the dodecyl sulfate anion …Web

قرأ أكثر

Physicochemical Studies on the Interaction between Sodium Dodecyl

The surface activity and the process of micellization of sodium dodecyl sulfate (SDS) with methylene blue () in a methanol-water mixed solvent system were investigated by tensiometry and conductometry at 298.15 K. The measurements of surface tension and conductivity of SDS- complex were carried up to 0.3 volume fractions of …Web

قرأ أكثر

Thermodynamics of Lipid Membrane Solubilization by Sodium …

Thermodynamics of Lipid Membrane Solubilization by Sodium Dodecyl Sulfate - PMC Journal List Biophys J v.90 (12); 2006 Jun 15 PMC1471870 As a library, …Web

قرأ أكثر

Solubilization and separation of o-toluidine and tricyclazole in sodium

The solubilization laws of pollutants in micelles and their separation efficiency are very important in the successfully efficient application of micellar enhanced ultrafiltration (MEUF). The solubilization behavior of o-toluidine (OT) and tricyclazole (TC) into sodium dodecyl sulfate (SDS) micelles in MEUF was studied using nonlinear equation sets for …Web

قرأ أكثر

(PDF) Solubilization of Nile Red in Micelles and

Abstract and Figures. A spectrophotometric study of the solubilization and aggregation of the Nile red dye (NR) in premicellar and micellar aqueous solutions of sodium dodecyl sulfate (SDS) was ...Web

قرأ أكثر

Drug solubilization effect of lauroyl-L-glutamate

This article proposes a new technique for the solubilization of poorly soluble drugs using lauroyl-L-glutamate, which is one of the amino acid detergents, with additional small additives. ... The synergistic effect was not observed by sodium dodecyl sulphate with arginine. The solubilizing system can be applied to other drugs because of the low ...Web

قرأ أكثر

Purification of RNA by SDS Solubilization and Phenol Extraction

INTRODUCTION. This protocol describes a method for RNA purification by sodium dodecyl sulfate (SDS) solubilization and phenol extraction. It is of wide utility and is used routinely to deproteinize RNAs in biological material that has been solubilized in SDS, an …Web

قرأ أكثر

Mechanisms of Solubilization of Mixed Liposomes: Preferential

The solubilization dynamics of dimyristoylphosphatidylcholine (DMPC) liposomes, as induced by sodium dodecyl sulfate (SDS), were investigated and it was found that solUBilization occurred not only via a uniform dissolution of the liposome membrane, but also via a dissolution involving the rapid motion of the Liposome, or via …Web

قرأ أكثر

Sodium dodecyl sulfate | C12H25NaO4S | CID …

Sodium dodecyl sulfate is an organic sodium salt that is the sodium salt of dodecyl hydrogen sulfate. It has a role as a detergent and a protein denaturant. It contains a dodecyl sulfate. Sodium Lauryl Sulfate (SLS) …Web

قرأ أكثر

The Mechanism of Vesicle Solubilization by the Detergent Sodium Dodecyl

Abstract. Membrane solubilization by sodium dodecyl sulfate (SDS) is indispensable for many established biotechnological applications, including viral inactivation and protein extraction. Although ...Web

قرأ أكثر

Sodium dodecyl sulfate solution

Sodium dodecyl sulfate solution Product Number L 4522 Store at Room Temperature Product Description Molecular Formula: C12H25NaO4S (SDS salt) Molecular Weight: …Web

قرأ أكثر

Effects of Sodium Dodecyl Sulfate on Mixed Liposome …

Ability of sodium dodecyl sulfate to transiently stabilize a phospholipid molecular layer. Thin Solid Films 2016, 615, 215-220. …Web

قرأ أكثر

Impact of Micellar Surfactant on Supersaturation and Insight into

The solubilization mechanism as a function of atazanavir concentration was probed using fluorescence spectroscopy. ... Curcumin was purchased from Spectrum Chemical Mfg. Corp. (Gardena, CA). Sodium dodecyl sulfate (SDS), pyrene carboxaldehyde, pyrene, dodecyl pyridinium chloride, deuterium oxide were obtained …Web

قرأ أكثر

Micellization behaviour of sodium dodecyl sulfate in different

The aggregation behavior of sodium dodecyl sulfate (SDS) was studied in an aqueous solution in the presence of increasing concentrations of selected sodium 1-alkylsulfonates, namely sodium 1-octanesulfonate, sodium 1-decanesulfonate, and sodium 1-dodecanesufonate. ... Furthermore, the solubilization power (SP) of both surfactants …Web

قرأ أكثر

The Mechanism of Vesicle Solubilization by the Detergent Sodium Dodecyl

The findings support a general mechanism of detergent-induced membrane solubilization and expect the framework of correlative biophysical technologies presented here will form a general platform for elucidating the complex kinetics of membrane perturbation induced by a wide variety of surfactants and disrupting agents. Membrane …Web

قرأ أكثر

Mechanism of mixed liposome solubilization in the presence of sodium

Solubilization of liposomes made up of 1 mM phosphatidyl choline only by sodium dodecyl sulfate as measured by optical density is given in Fig. 1. Here, the dependence of liposome solubilization on SDS concentration for different interaction times is shown. After 15 min. of interaction, about 35% of the liposome has been solubilized by …Web

قرأ أكثر

The Mechanism of Vesicle Solubilization by the Detergent Sodium Dodecyl

Membrane solubilization by sodium dodecyl sulfate (SDS) is indispensable for many established biotechnological applications, including viral inactivation and protein extraction. Although the ensemble thermodynamics have been thoroughly explored, the underlying molecular dynamics have remained inaccessible, owing to major limitations of ...Web

قرأ أكثر

Qualitative and quantitative methods detection of SDS based on …

Sodium dodecyl sulfate (SDS) is the most widely used anionic surfactant. Its frequent use causes environmental pollution and negative effects on living organisms (even at low concentrations ≈ 20 ...Web

قرأ أكثر

New mass-spectrometry-compatible degradable surfactant for …

Conventional surfactants such as sodium dodecyl sulfate (SDS), the preferred surfactant for protein solubilization, are not compatible with MS. Herein, we have screened a library of surfactant-like compounds and discovered an MS-compatible degradable surfactant (MaSDeS) for tissue proteomics that solubilizes all categories of …Web

قرأ أكثر

Solubilization of Nile Red in Micelles and Protomicelles of Sodium

Abstract. A spectrophotometric study of the solubilization and aggregation of the Nile red dye (NR) in premicellar and micellar aqueous solutions of sodium dodecyl sulfate (SDS) was carried out. The experiments were conducted both with saturated solutions of NR under conditions of thermodynamic equilibrium of the solution with a dye precipitate ...Web

قرأ أكثر

(PDF) Evaluation of sodium deoxycholate as solubilization buffer for

In conclusion, sodium deoxycholate is applicable in the solubilization of proteins extracted from oil palm mesocarps with higher efficiency compared to urea/thiourea/CHAPS buffer. The sodium ...Web

قرأ أكثر

Micellar solubilization of poorly water-soluble drugs: effect of

Drug solubilization increases linearly with the increase of hydrophobic chain length for all studied surfactant types. Addition of 1-3 ethylene oxide (EO) units in the head group of dodecyl sulfate surfactants reduces significantly the solubilization of both studied drugs and decreases linearly the solubilization locus polarity of fenofibrate.Web

قرأ أكثر

Studies on the phase behavior of the microemulsions formed by sodium

The middle-phase behavior for the quaternary system of sodium dodecyl sulfonate (AS) (sodium dodecyl sulfate, ... Effect of interfacial alcohol concentrations on oil solubilization by sodium dodecyl sulfate micelles. J. Colloid Interface Sci., 228 (2000), pp. 18-23. View PDF View article View in Scopus Google ScholarWeb

قرأ أكثر

Changes in phospholipid bilayers caused by sodium dodecyl …

The interaction of mixtures of sodium dodecyl sulfate (SDS) and oxyethylenated nonylphenol (30 mol of ethylene oxide) [NP(EO) 30] with phosphatidylcholine liposomes was investigated.Permeability alterations were detected as a change in 5(6)-carboxyfluorescein (CF) released from the interior of vesicles, and bilayer solubilization was measured as a …Web

قرأ أكثر

The Mechanism of Vesicle Solubilization by the Detergent …

The solubilization of lipid membranes by the anionic detergent sodium dodecyl sulfate (SDS) has found vast utility across the pharmaceutical and biological sectors with far …Web

قرأ أكثر

Sodium dodecyl sulfate

as a protein and lipid solubilization reagent. As a general rule for the solubilization of proteins, SDS should be used at its critical micelle concentration.2 SDS is also a …Web

قرأ أكثر

Oil solubilization in sodium dodecylbenzenesulfonate micelles: …

The maximum solubilization capacity can be obtained from the intersection of the two straight lines at low and high 1-hexene concentration. Below the maximum solubilization capacity, micelles are still capable of solubilizing some further1-hexene; this phenomenon is called "swollen micelles" according to Kralchevsky's research [33], [34 ...Web

قرأ أكثر

Solubilization of Nitrogen Heterocyclic Compounds Using Different

In order to develop surfactant-enhanced remediation for nitrogen heterocyclic compounds (NHCs) (aniline, indole, and quinolone), the solubilization properties of micellar solutions of five surfactants, namely sodium dodecyl sulfate (SDS), rhamnolipid (RL), polysorbate (Tween 80), sorbitan monolaurate (Span 20), and iso-octyl phenoxy …Web

قرأ أكثر

Thermodynamics of sodium dodecyl sulfate partitioning into lipid

Complete membrane solubilization requires a ratio of 2.2 mol SDS bound per mole of total lipid at 56 degrees C. The corresponding equilibrium concentration of SDS free in …Web

قرأ أكثر

Thermodynamics of sodium dodecyl sulfate partitioning into lipid

The partition equilibria of sodium dodecyl sulfate (SDS) and lithium dodecyl sulfate between water and bilayer membranes were investigated with isothermal titration calorimetry and spectroscopic methods (light scattering, (31)P-nuclear magnetic resonance) in the temperature range of 28 degrees C to 56 degrees C.Web

قرأ أكثر

Evaluation of sodium deoxycholate as solubilization buffer for oil …

Removal of sodium dodecyl sulphate, urea, CHAPS using filter-aided sample preparation (FASP) and zip tips [66–68] still resulted in interferences to liquid chromatography runs and mass spectrometric analysis [68, 69]. The limitation of the utilization of SDC in protein solubilization, which was observed from the study, was the …Web

قرأ أكثر

Solubilization and Refolding of Inclusion Body Proteins

The process of recovering bioactive proteins from IBs consists of solubilization of IB aggregates using denaturants, followed by refolding of the solubilized protein. ... Chaotropic agents such as urea and GdnHCl, in the presence of low concentration of detergents such as sodium dodecyl sulfate (SDS), sodium …Web

قرأ أكثر

Sodium dodecyl sulfate solution

as a protein and lipid solubilization reagent. In general, for solubilization of proteins, SDS should be used at its critical micelle concentration.2 SDS is also ... Sodium Dodecyl Sulfate from Proteins. Anal. Biochem., 91(1), 230-235 (1978). 8. Hayashi, K., A Rapid Determination of Sodium Dodecyl Sulfate with Methylene Blue. Anal.Web

قرأ أكثر

Purification of RNA by SDS solubilization and phenol extraction

This protocol describes a method for RNA purification by sodium dodecyl sulfate (SDS) solubilization and phenol extraction. It is of wide utility and is used routinely to deproteinize RNAs in biological material that has been solubilized in SDS, an ionic detergent that dissolves membranes, disrupts protein-nucleic acid interactions, and inactivates …Web

قرأ أكثر

Solubilization Behavior of Organic Mixtures in Optimum Winsor …

Solubilization selectivity and synergism behavior were investigated for the optimum Winsor type III sodium dodecyl sulfate (SDS) microemulsion systems of organic mixtures. The organic mixtures used include toluene and 1 of the following 3 normal alkanes: n -octane, n -heptane, and n -hexane.Web

قرأ أكثر

Mechanism for the complex micellar system of sodium dodecyl …

In this study, surfactants sodium dodecyl sulfate (SDS) and octylphenol ethoxylate OPE9 (Triton X-100) were selected to better understand the mechanism of the surfactant solution to solubilize methane from a microscopic aspect. ... The mixed SDS and Triton X-100 solution was proven greater at methane solubilization than a single …Web

قرأ أكثر

Solubilization of two organic dyes by anionic, cationic and …

A straight chain alkyl tail seemed to be better than an alkylaryl tail, as judged from the comparison of solubilization power for the pairs sodium dodecyl sulphate (SDS)/sodium dodecyl benzene ...Web

قرأ أكثر

Effects of Sodium Dodecyl Sulfate on Mixed Liposome Solubilization

The solubilization process of sorbitan monooleate (Span 80) niosomes, formed by sonication, by addition of the anionic surfactant sodium dodecyl sulfate (SDS) was studied in this work. The main … ExpandWeb

قرأ أكثر